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KMID : 0604520080340030157
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Journal of the Society of Cosmetic Scientists of Korea
2008 Volume.34 No. 3 p.157 ~ p.165
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Component Analysis of Suaeda asparagoides Extracts
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Yang Hee-Jung
Park Soo-Nam
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Abstract
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In the previous study, the anti-oxidant activity of oxtract/fraction of Sueada aspparagoides(SA) and the stability test for the cream containing SA extract were investigated respectively[1,2]. In this study, the components of SA extract were analyzed by TLC, HPLC, and LC/ESI-MS/MS, -NMR. TLC chromatogram of ethyl acetate fraction of SA extract revealed 5 bands . HPLC chromatogram of aglycone fractions obtained from deglycoylation reaction of ethyl acetate fraction showed 2 bands (SAA 2 and SAA 1), which were identified as quercetin (composition ratio, 16.88%) and kaempferol (83.12%) in the order of elution time. Among 5 bands of TLC chromatogram, 4 bands also were Identified as kaempferol-3-O-glucoside (SA 2), quercetin-3-O-glucoside (SA3), kaempferol-3-O-rutinoside (SA 4), quercetin-3-O-rutinoside (SA 5) by LC/ESI-MS/MSMS/MS. respectively. The spectrum generated for SAA 1 by LC/ESI-MS/MS in the negative ion mode also gave the ion corresponding to the deprotonated aglycone (285m/z), the -NMR spectrum contained signals [ 6.19 (1H, d, J=1.8Hz, H-6), 6.44 (1H, d, J=1.8Hz, H-8), 6.92 (2H, d, J=9.0Hz, H-3’, 5’), 8.04 (2H, d, J=9.0Hz, H-2’, 6’, thus SAA 1 was identified as kaempferol. SAA 2 yielded the deprotonated agycone ion (301m/z), -NMR spectrum showed signals [ 6.20 (1H, d, J=2.0Hz, H-6), 6.42 (1H, d, J=2.0Hz, H-8), 6.90 (1H, d, J=8.6Hz, H-5’), 7.55 (1H, dd, J=8.6, 2.2Hz, H-6’), 7.69 (1H, d, J=2.2Hz, H-2’, thus SAA 2 was Identified as quercetin. In conclusion, with the anti-oxidant activity and the stability test reported previously, component analysis of SA extracts could be applicable to new cosmeceuticals.
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KEYWORD
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Suaeda asparagoides, flavonoids, LC/ESI-MS/MS, component analysis, NMR
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